An In Vitro Model of Diabetic Retinal Vascular Endothelial Dysfunction and Neuroretinal Degeneration

Table 2

Apoptotic index in RGCs at 24 h, 48 h, and 72 h.

Groups	24 h	value	48 h	value	72 h	value
	Apoptotic index		Apoptotic index		Apoptotic index
Con	0.042 (0.028-0.047)	—				—
G25	0.069 (0.031-0.112)	0.171^b		0.006^a		0.182^a
G50	0.108 (0.071-0.144)	0.015^b		0.284^a		0.003^a
G100	0.120 (0.061-0.256)	0.003^b		0.006^a		0.016^a
G150	0.148 (0.079-0.184)	0.001^b		0.002^a	0.291 ± 0.124	0.001^a
M25	0.049 (0.025-0.082)	0.691^b		0.329^a		0.893^a
M50	0.036 (0.024-0.059)	0.691^b		0.221^a		0.856^a
M100	0.050 (0.030-0.065)	0.354^b		0.054^a		0.733^a
M150	0.056 (0.030-0.082)	0.270^b		0.455^a		0.918^a

Statistically significant: p <0.05. According to the type of data and the data distribution, ^aindependent-sample -test and the ^bMann-Whitney test were applied. Con-5.5, G25-G150: concentration of glucose (5.5, 25, 50, 100, and 150 mmol/l, respectively); M25-M150: concentration of M-mannitol (19.5, 44.5, 94.5, and 133.5 mmol/l, respectively).