Pigment Epithelium-Derived Factor-Loaded PEGylated Nanoparticles as a New Antiangiogenic Therapy for Neovascularization

Table 2

Summary of proliferation effects of PEDF and NP-PEG-PEDF combined with VEGF-treated HUVECS under high glucose (30 mmol/L).

Time points		10% FBS	VEGF (20 ng/mL)	NP-PEG	PEDF			NP-PEG-PEDF
				1000 ng/mL	1000 ng/mL	100 ng/mL	10 ng/mL	1000 ng/mL	100 ng/mL	10 ng/mL
24 h	Mean	1	123.61	99.19	79.83	74.57	84.48	57.32	59.48	62.07
	SEM		2.98	3.14	1.67	3.54	3.2	2.3	2.89	1.41
48 h	Mean	1	114.65	110.37	91.19	96.11	97.12	63.12	68.12	62.45
	SEM		6.56	9.24	7.23	5.29	5.81	4.32	5.27	6.82
72 h	Mean	1	115.21	105.25	87.68	90.58	91.03	73.33	79.71	87.82
	SEM		7.89	7.48	6.22	2.73	4.14	6.56	3.59	4.23