Klotho Ameliorates Podocyte Injury through Targeting TRPC6 Channel in Diabetic Nephropathy

<div>KL overexpression inhibited HG-induced podocyte injury. Podocytes were exposed to the adenovirus that expressed KL for 24 h and afterwards cultured with HG for extra 48 h. (a) The efficiency of Ad-NC and Ad-KL adenovirus infection examined by GFP fluorescence. (b) Representative western blots and (c) densitometric analyses of KL, Synaptopodin, and TRPC6 expression as aforementioned. (c) Typical images of phalloidin staining as aforementioned. Scale bars, 10 <i>μ</i>m. (d) Quantified outcomes in (c). <span class="nowrap"><svg height="8.55521pt" id="M26" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 503C384 579 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27V0H384Z"></path></g></svg> versus HG group.</div>

Journal of Diabetes Research

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Figure 2

Figure 2: Klotho Ameliorates Podocyte Injury through Targeting TRPC6 Channel in Diabetic Nephropathy 