Klotho Ameliorates Podocyte Injury through Targeting TRPC6 Channel in Diabetic Nephropathy

Figure 5

Decrease of KL expression in the glomeruli of db/db animals. (a) Glomerular KL expression levels in Ju CKD Glom data from Nephroseq. (b) Immunofluorescence staining unveiled that KL was colocalized with Podocin and Nephrin in podocytes. (c) Representative images of PAS, TEM, and immunofluorescence staining for WT-1, Synaptopodin, and KL in db/m and db/db animals. Scale bars for PAS, WT-1, Synaptopodin, and KL pictures, 10 μm; Scale bars for TEM, 10 μm. (d) Semiquantitation of glomerular size, glomerulosclerotic index, foot process width, quantity of podocytes positive for WT-1 per glomerulus, and Synaptopodin and KL expression areas in (c). (e) Typical WB and (f) densitometric analyses of KL, Synaptopodin, and TRPC6 expression in db/m and db/db animals. . versus db/m group.

(a)

(b)

(c)

(d)

(e)

(f)