Klotho Ameliorates Podocyte Injury through Targeting TRPC6 Channel in Diabetic Nephropathy

Figure 6

Renal KL overexpression inhibited TRPC6 and mitigated podocyte function disorder in db/db animals. (a) Schematic diagram of experiment process. 12-week-old db/db animals were inoculated with adenovirus expressing KL or negative control adenovirus via tail veins. Urine was harvested on 0 d, 7 d, 14 d, and 28 d postinjection with adenovirus, and renal samples were harvested on 28 d postinjection with adenovirus. (b) Representative western blots and (c) densitometric analyses of KL, Synaptopodin, and TRPC6 expression in the indicated groups. (d) Urinary ACR in the animals on days 0, 7, 14, and 28 postinjection with Ad-NC or Ad-KL. (e) Representative images of PAS, TEM, and immunofluorescence staining for WT-1 and Synaptopodin in the indicated groups. Scale bars for PAS staining, WT-1, and Synaptopodin pictures, 10 μm; Scale bars for TEM, 10 μm. (f) Semiquantitation of glomerulosclerotic index, foot process width, quantity of podocytes positive for WT-1 per glomerulus, and Synaptopodin expression region in (e). . versus db/m group; ^## versus db/db group.

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