San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway

Figure 3

Effects of SHYS on PINK1/Parkin-mediated mitophagy in DKD model rats. The changes in morphology of the mitochondria in the kidney in each group were observed by transmission electron microscopy and the levels of proteins related to PINK1/Parkin-mediated mitophagy were detected by western blot. (a, b) Transmission electron microscopy showed that SHYS treatment improved the morphology of mitochondria in kidney (M indicated mitochondria and MS indicated mitophagosomes) () (a). The percentage of fragmented mitochondria in transmission electron microscopy was calculated. SHYS treatment decreased the percentage of fragmented mitochondria in the kidney (b). (c, d) SHYS treatment decreased the levels of VDAC1, TOM20, and COXIV in kidney. (e, f) SHYS treatment increased the levels of PINK1 and Parkin in the kidney. (g, h) SHYS treatment increased the level of LC3-II and decreased the level of p62 in kidney. ^#: compared with the control group; ^##: compared with the control group; : compared with the model group; : compared with the model group.

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