Beta-Hydroxybutyric Acid Inhibits Renal Tubular Reabsorption via the AKT/DAB2/Megalin Signalling Pathway

Figure 3

Effects of different concentrations of BOHB and acetoacetate on endocytosis and megalin expression of HK-2 cells. (a) Quantification of FITC-BSA uptake in HK2 cells in different concentrations of BOHB. (b) Quantification of FITC-BSA uptake in HK2 cells in the indicated pH level. pH 7.1 represents 0 mmol/l BOHB; pH 7.0 represents 0.5 mmol/l BOHB; pH 6.8 represents 1 mmol/l BOHB; pH 6.7 represents 2 mmol/l BOHB; pH 6.6 represents 3 mmol/l BOHB; pH 6.5 represents 4 mmol/l BOHB. (c) Quantification of FITC-BSA uptake in HK2 cells in different concentrations of BOHB when pH was adjusted to 7.4. (d) Representative western blotting images and quantification of megalin in different concentrations of BOHB. (E) Representative western blotting images and quantification of megalin in different concentrations of AcAc. AcAc, acetoacetate; BOHB, beta-hydroxybutyric acid. Data are expressed as . Represents ; represents .

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