Regulation of Glucose Insulinotropic Peptide and Intestinal Glucose Transporters in the Diet-Induced Obese Mouse

Figure 1

Effect of high-fat diet on SGLT1 function in mouse proximal jejunum. SGLT1 function was measured both as a function of O-methyl-glucose- (OMG-) induced Isc and as OMG fluxes in animals fed normal (a and c) or high-fat (b and d) diet. Isc was measured 45 minutes after either mucosal or serosal addition of OMG (10 mM) in normal (a) and 5 months high-fat (b) diet fed animals. To measure OMG fluxes, 10 mM OMG was added to either mucosal or serosal bath. Forty-five minutes following OMG addition, 500 μl samples were taken from the opposite side to measure mucosal to serosal (m-s) and serosal to mucosal (s-m) OMG fluxes in normal (c) and 5 months high-fat (d) diet fed animals. Isoosmolality was maintained by adding 10 mM mannitol to the bath opposite to the OMG bath. Results presented represent the from 4 tissues obtained from 4 different mice. —compared to basal control.

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