Regulation of Glucose Insulinotropic Peptide and Intestinal Glucose Transporters in the Diet-Induced Obese Mouse

Figure 5

SGLT1 and GLUT2-specific protein expression in animals fed a normal and high-fat diet after 5 months (a and b) and 9 months (c and d). (a and c) Biotinylation and western blots of GLUT2 (a) and SGLT1 (b) proteins are shown. Total lanes are total cell homogenates (no biotinylation) that includes both the apical, basolateral, and internal membranes of villus cells and serves as a control. Jejunum from animals at five months of a normal and high-fat diet are used. Each glucose (+glu) and glucose and GIP (+glu+GIP) blots represent cell homogenates prepared from biotinylations from jejunal mucosa along with a total homogenate each from a single animal. Since actin is not present in the membranes, e-cadherin loading controls are used instead. A representative blot is shown from 4 different animals. (b and d) Densitometry of 4 representative blots is shown. Results presented represent from four mouse jejunums. —compared to control. ⁺—compared to control.

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