Long Noncoding RNA ENSG00000254693 Promotes Diabetic Kidney Disease via Interacting with HuR

Figure 2

The effect of lncRNA 254693 in podocyte inflammation and apoptosis. (a) The expression of lncRNA 254693 in human podocytes indicated by FISH assay.  μm, . (b) The expression of lncRNA 254693 in human podocytes measured by qRT-PCR. versus the LG group, . (c) 5 and 3 rapid amplification of cDNA end (RACE) assays in human podocytes to detect the whole sequence of lncRNA 254693. (d) Northern blot analysis to confirm the length and expression of lncRNA 254693. (e) The level of lncRNA 254693 after silencing of lncRNA 254693 in podocytes. versus the control group, . (f) The protein levels of IL-6, TNF-α, and cleaved caspase-3 in response to the treatment of LG, HG, HG+si-NC, and HG+si-254693. vs. the LG group, ^# vs. the HG group, . (g) The mRNA levels of IL-6 and TNF-α in response to the treatment of LG, HG, HG+si-NC and HG+si-254693. vs. the LG group; ^# vs. the HG group, . LG: low-glucose; HG: high-glucose; HO: hyperosmolality; NC: negative control.

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