Long Noncoding RNA ENSG00000254693 Promotes Diabetic Kidney Disease via Interacting with HuR

Figure 4

The interaction between lncRNA 254693 and HuR. (a) Western blot assay for the expression of HuR in human podocytes after lncRNA 254693 knockdown, . (b) qRT-PCR for the mRNA level of HuR in human podocytes after lncRNA 254693 knockdown. vs. the LG group, ^# vs. the HG group, . (c) Immunofluorescence and in situ hybridization double staining of human podocytes with different conditions to detect lncRNA 254693 (red) and HuR (green) expression and colocalization.  μm, . (d, e) The efficiency of HuR knockdown was confirmed by decreased protein and mRNA expression level. vs. the control group, . (f) The expression of lncRNA 254693 in human podocytes after HuR knockdown. vs. the LG group; ^# vs. the HG group, . (g) The effect of HuR on lncRNA 254693 RNA stability. Human podocytes were transfected with HuR siRNA and actinomycin D (5 μg/mL) was then used to treat cells at different time points. RNA was detected by qRT-PCR. vs. the NC group, . (h) Relative enrichment of lncRNA 254693 associated with HuR in human podocytes detected by anti-HuR RIP (IgG as negative control). vs. IgG, ^# vs. the HG group.

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