Quantifying Podocyte Number in a Small Sample Size of Glomeruli with CUBIC to Evaluate Podocyte Depletion of db/db Mice

Figure 2

A limited number of glomeruli may represent the overall podocyte number in C57 mice. (a) The podocyte number per glomerulus of 1017 glomeruli in five C57 mice was quantified with the Imaris software through identifying 3D reconstruction images of podocytes per glomerulus and exhibited in chart. The mean of podocyte number per glomerulus was calculated from 1017 glomeruli. The sum of podocyte number from 1017 glomeruli was used as the numerator, which was divided by the total number of glomeruli, 1017. (b) No statistical significance in podocyte number per glomerulus was observed among five C57 mice, which proposed stability distribution of podocyte number per glomerulus within C57 mice. Data are expressed as . (c) The tables displayed the sample size of glomeruli needed to represent the whole sample size with taking 5%, 7.5%, and 10% of the mean podocyte number per glomerulus as the statistical allowable error and setting total confidence level as 95% by the PASS software.

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