Quantifying Podocyte Number in a Small Sample Size of Glomeruli with CUBIC to Evaluate Podocyte Depletion of db/db Mice

<div>Characterization of db/db mice. (a) db/db mice (<span class="nowrap"><svg height="9.46863pt" id="M7" style="vertical-align:-1.11981pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 9.46863" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M531 285V335L57 550V494L473 310L57 127V71L531 285ZM531 -40V10H57V-40H531Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 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27V0H384Z"></path></g></svg> for db/db mice by the age of 8 w, 12 w, and 16 w versus the control group. (b) PAS-stained kidney sections revealed increased glomerular mesangial matrix in db/db mice compared with the control group. Shown are representative images from at least three mice per group with similar results (scale bar: 100 <i>μ</i>m in all images of (b)).</div>

Journal of Diabetes Research

fig3

Figure 3

Figure 3: Quantifying Podocyte Number in a Small Sample Size of Glomeruli with CUBIC to Evaluate Podocyte Depletion of db/db Mice 