Quantifying Podocyte Number in a Small Sample Size of Glomeruli with CUBIC to Evaluate Podocyte Depletion of db/db Mice

Figure 4

Three-dimensional visualization of podocytes in control and DKD glomeruli. Representative images of podocytes from the control group and db/db mice labeled with antibody to WT-1 (red) by the CUBIC method. Podocytes are visible as higher density of WT-1-stained nuclei is observed within the control group than db/db mice. Shown are representative images from three mice of every group with similar results (scale bar: 50 μm).