ceAF Ameliorates Diabetic Wound Healing by Alleviating Inflammation and Oxidative Stress via TLR4/NF-κB and Nrf2 Pathways

Figure 1

ceAF was able to suppress inflammation and oxidative stress via TLR4/NF-κB and Nrf2 pathways in LPS-stimulated RAW 264.7 cells via TLR4/NF-κB and Nrf2 pathways. (a) The viability of RAW 264.7 cells exposed to ceAF at various concentrations. (b) The expression levels of CD206, Arg-1, iNOS, IL-1β, IL-6, and TNF-α were assessed by RT-qPCR. (c) The levels of ROS SOD, GSH-Px, MDA, and MPO were measured. (d) WB results of the influence of ceAF on macrophage markers and TLR4/NF-κB axis. CD206, Arg-1, iNOS, IL-1β, IL-6, TLR4, TNF-α, NF-κB p65, pIκB Nrf2, HO-1, and NQO1 were tested, and GAPDH served as a standard reference. (e) Bands from the WB in (d) were analyzed by densitometry. , . NC: negative control; , , .

(a)

(b)

(c)

(d)

(e)