Use of a Combined Technology of Ultrasonication, Three-Phase Partitioning, and Aqueous Enzymatic Oil Extraction for the Extraction of Oil from Spirogyra sp.

Table 2

Effect of temperature on papain assisted oil extraction (by TPP). The sonicated aqueous suspension of algae containing 2 g of crude papain (100 units) was incubated at pH 6 at different temperatures for 6 h as optimized earlier and it was subjected to three-phase partitioning. The control was the suspension without enzyme. The yield of the oil in each case was noted and expressed in terms of %, w/w, dry weight of algae.

Entry	Temp	Control (TPP); (yield %, w/w)	Papain + TPP; (yield %, w/w)
1	30°C	9	14
2	40°C	11	18
3	50°C	12	22
4	60°C	12.5	27
5	70°C	13	25