Assessment of the Presence of Resistance Genes Detected from the Environment and Selected Food Products in Benin

Table 2

Primers of virulence genes and desired ESBL genes in the genome of isolates.

Genes	Primer	Sequence (5’-3’)	Amplicon size (bp)	Reference (s)
Virulence
PapC	F	GACGGCTGTACTGCAGGGTGGCG	328	[3]
	R	ATATCCTTTCTGCAGGGATGCAATA
fimH	F	TACTGCTGATGGGCTGGTC	640
	R	GCCGGAGAGGTAATACCCC
iss	F	GGCAATGCTTATTACAGGATGTGC	260
	R	GAGCAATATACCCGGGCTTCC
Biofilm	F	GATTCAATTTTGGCGATTCCTGC	225
	R	TAATGAAGTCATTCAGACTCATCC
Stx1	F	CGCTGAATGTCATTCGCTCTGC	302
	R	CGTGGTATAGCTACTGTCACC
Stx2	F	CCTCGGTATCCTATTCCCGG	516
	R	CTGCTGTGACAGTGACAAAACGC
ESBL
blaCTX-M1	F	GGTTAAAAAATCACTGCGTC	863	[4]
	R	TTGGTGACGATTTTAGCCGC
blaCTX-M2	F	ATGATGACTCAGAGCATTCG	865
	R	TGGGTTACGATTTTCGCCGC
blaCTX-M9	F	ATGGTGACAAAGAGAGTGCA	869
	R	CCCTTCGGCGATGATTCTC
blaCTX-M15	F	CACACGTGGAATTTAGGGACT	995
	R	GCCGTCTAAGGCGATAAACA
blaTEM	F	ATGAGTATTCAACATTTCCGC	856
	R	CAATGCTTAATCAGTGAGG
blaSHV	F	AAGATCCACTATCGCCAGCAG	230
	R	ATTCAGTTCCGTTTCCCAGCGG