Network Pharmacology Analysis and Experimental Pharmacology Study Explore the Mechanism of Asparagus against Glioblastoma

Figure 6

Inhibitory effect of ASP on GBM cells. (a) The CCK8 assay was used to determine the IC50s of SVGP12, U87, and U251 cell lines treated with ASP for 72 hours. (b) U87 and U251 cell lines were treated with ASP as indicated for 7 days and then crystal violet stained. A western blot was performed to detect the expression of AKT1, p-AKT, p-S6RP, and p-4EBP1 in ASP-treated U87 (c) and U251 (d) cells. β-actin was used as a normalization standard. The mean ± S. E. M. for three independent experiments is shown. ; (Student’s t-test).

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