Network Pharmacology Analysis and Experimental Pharmacology Study Explore the Mechanism of Asparagus against Glioblastoma

Figure 7

p53-dependent apoptosis is induced by ASP activation. (a) Western blot was performed to detect the expression of P53 in ASP-treated GBM cells. (b) Pathways Venn diagram. The arrow indicates the p53-dependent apoptosis genes. Western blot was performed to detect apoptosis-related proteins in ASP-treated U87 (c) U251 (d) cells. U87 (e) and U251 (f) cells were stained with Hoechst 3342/PI to detect the effect of ASP on apoptosis. Scale bar, 200 μm. (g) A flow cytometric analysis of annexin V and propidium iodide (PI) stained cells was conducted to evaluate apoptosis. β-actin was used as a normalization standard. Mean ± S. E. M. for three independent experiments is shown. ; (Student’s t-test).

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