Low-Dose Alcohol Improves Lipid Metabolism through Store-Operated Ca²⁺ Channel-Induced PPARγ Expression in Obese Mice

Figure 4

Low-dose alcohol consumption upregulated PPARγ expression. (a) Western blots of PPARγ, APN, and FABP4 in liver tissues of mice. (b) Quantification of PPARγ, APN, and FABP4 protein levels in liver tissues as in (a). (c) Hepatic mRNA levels of PPARγ, APN, and FABP4 were quantified by RT-qPCR. In MTT assay, L02 cells were treated with alcohol (0, 10, 20, 50, 100, and 200 mM) for 24 h. (d) Determination of cell viability by MTT in L02 cells (n = 6). L02 cells were treated with alcohol (0, 10, 20, and 50 mM) for 24 h. (e) Western blots of PPARγ, APN, and FABP4 in L02 cells. (f) Quantification of PPARγ, APN, and FABP4 protein levels in L02 cells as in (e). (g) mRNA levels of PPARγ, APN, and FABP4 were quantified by RT-qPCR in L02 cells. n = 3,  < 0.05 vs. ND group; ^# < 0.05 vs. HFD group.

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