Low-Dose Alcohol Improves Lipid Metabolism through Store-Operated Ca²⁺ Channel-Induced PPARγ Expression in Obese Mice

Figure 6

Low-dose alcohol consumption upregulated PPARγ by SOCs. L02 cells were pretreated with EGTA (10 mM) for 1 h and then treated with alcohol (20 mM) for 24 h. (a) Western blots of PPARγ, APN, and FABP4. (b) Quantification of PPARγ, APN, and FABP4 protein levels as in (a). (c) PPARγ, APN, and FABP4 mRNA levels were measured by RT-qPCR. L02 cells were treated with alcohol (0, 10, 20, and 50 mM) for 24 h. (d) Western blots of STIM1 and Orai1. (e) Quantification of STIM1 and Orai1 protein levels as in (d). (f) STIM1 and Orai1 mRNA levels were measured by RT-qPCR. L02 cells were pretreated with 2-APB (50 mM) for 1 h and then treated with alcohol (20 mM) for 24 h. (g) Western blots of PPARγ, APN, and FABP4. (h) Quantification of PPARγ, APN, and FABP4 protein levels as in (g). (i) PPARγ, APN, and FABP4 mRNA levels were measured by RT-qPCR. n = 3,  < 0.05 vs. control or 0 mM Alc; ^# < 0.05 vs. Alc.

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