Hesperidin Induced HePG-2 Cell Apoptosis through ROS-Mediated p53/Bcl-2/Bax and p-mTOR Signaling Pathways

Figure 3

Translocation of phosphatidylserine induced by different concentrations of hesperidin in HepG-2 cells. Normal cells are depicted in the lower left quadrant, late apoptotic cells are depicted in the upper right quadrant, and early apoptotic cells are depicted in the lower right quadrant. The upper right quadrant indicated cells in the early stage of apoptosis, and the lower right quadrant shows cells in the late stage of apoptosis or necrosis. Different concentration gradients of hesperidin include 40 μM, 80 μM, 160 μM, 320 μM, and 640 μM. It was observed that the number of apoptotic cells increased with the increase of hesperidin concentration.