Hesperidin Induced HePG-2 Cell Apoptosis through ROS-Mediated p53/Bcl-2/Bax and p-mTOR Signaling Pathways

<div>Caspase-3 and PARP-mediated apoptosis induced by Hesperidin in HepG-2 cells. (a) The synthetic fluorogenic substrate analyzed HepG-2 cells treated with hesperidin and caspase-3 activity, which was measured at 405 nm using the microplate spectrophotometer (VersaMax). (b) The expression of PARP, C-PARP, Caspase-3, and C-Caspase-3 by western blot; <i>β</i>-actin was used as the loading control. (c) Bands were quantified by Image J and results are expressed as a percentage of control. Different concentration gradients of hesperidin include 40 <i>μ</i>M, 80 <i>μ</i>M, 160 <i>μ</i>M, 320 <i>μ</i>M, and 640 <i>μ</i>M. 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Journal of Food Biochemistry

fig5

Figure 5

Figure 5: Hesperidin Induced HePG-2 Cell Apoptosis through ROS-Mediated p53/Bcl-2/Bax and p-mTOR Signaling Pathways 