Hesperidin Induced HePG-2 Cell Apoptosis through ROS-Mediated p53/Bcl-2/Bax and p-mTOR Signaling Pathways

<div>ROS overproduction induced by hesperidin of HepG-2 cells. (a) Changes in intracellular ROS generation detected by measuring DCF fluorescence intensity, which was measured at 500 nm using the microplate spectrophotometer (VersaMax). (b) HepG-2 cells were incubated with ten <i>μ</i>M DCF-DA in PBS for 30 min and then treated with different concentrations of hesperidin under a phase-contrast microscope. The length of the scale plate in the footnote is 75 <i>μ</i>m. Different concentration gradients of hesperidin include 40 <i>μ</i>M, 80 <i>μ</i>M, 160 <i>μ</i>M, 320 <i>μ</i>M, and 640 <i>μ</i>M. 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Journal of Food Biochemistry

fig6

Figure 6

Figure 6: Hesperidin Induced HePG-2 Cell Apoptosis through ROS-Mediated p53/Bcl-2/Bax and p-mTOR Signaling Pathways 