Research Article

Potential Antioxidant Activity of Novel Antioxidant Peptides from Protein Hydrolysate of Peony Seed Dreg in Chemical and H2O2-Induced RAW264.7 Cells

Table 3

The antioxidant activities of the synthesized peptides.

YFPFECCASLAPLYVSLKYFEMGSH

DPPH∙ scavenging activity (%)17.73 ± 0.9887.20 ± 1.0316.32 ± 1.4049.50 ± 1.50
∙OH scavenging activity (%)75.30 ± 1.0826.90 ± 0.7136.53 ± 0.4040.47 ± 0.2177.56 ± 1.40
ORAC (μmol TE/mg)1.50 ± 1.400.73 ± 1.502.52 ± 1.202.08 ± 1.20

The concentration of YFPF, YVSLK, and YFEM was 500 μg/ml, except in ORAC analysis, in which the concentration was 100 μg/ml. The concentration of ECCASLAPL was 500 μg/ml in the ∙OH scavenging activity analysis, 200 μg/ml in the DPPH∙ scavenging activity analysis, and 100 μg/ml in the ORAC analysis. The concentration of trolox was 25 μg/ml. Changes compared to GSH were analyzed for statistical significance, which is indicated with asterisks;  < 0.05.