Protective Activity of Alpha-Mangostin against UVB-Induced Injury in HaCaT Cells by Modulating the Ceramide and MAPK and NF-κB Signaling Pathways

Figure 1

The protective effect of α-MG in UVB-induced HaCaT cells. (a) HaCaT cells were treated with 2–20 μM α-MG for 2 h and subsequent complete medium DMEM replacement drug solution continued incubation for 24 h, and cytotoxicity of α-MG was evaluated by the MTT assay. (b) HaCaT cells were treated with 10–100 mJ/cm², and cytotoxicity of UVB was evaluated by the MTT assay. (c) HaCaT cells were pretreated with or without α-MG (2 μM) for 2 h and then exposed to UVB (50 mJ/cm²). At 24 h after UVB irradiation, cell viability was determined by the MTT assay. (d–f) MMP-1, MMP-2, and MMP-9 expressions were detected by RT-qPCR after α-MG pretreatment and then UVB irradiation ( vs. the control group; and vs. the UV group; n = 3-4; C: control group; U: UV group; T: treatment group).

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