Research Article
Protective Activity of Alpha-Mangostin against UVB-Induced Injury in HaCaT Cells by Modulating the Ceramide and MAPK and NF-κB Signaling Pathways
Figure 2
α-MG protects HaCaT cells against UVB-mediated apoptosis. (a, b) HaCaT cells were pretreated with or without α-MG (2 μM) for 2 h and then exposed to UVB (50 mJ/cm2). At 24 h after UVB irradiation, the cell apoptosis rate was determined by flow cytometry. (c, d) GAPDH as an internal control, the ratio of Bax/Bcl-2, and the expression levels of caspase-3 mRNA were presented. (e) After α-MG pretreatment and then UVB irradiation, Bcl-2, Bax, and caspase-3 were detected by western blot. (f, g) GAPDH as an internal control, the ratio of Bax/Bcl-2, and the expression levels of caspase-3 protein were presented (, , and vs. the control group; , , and vs. the UV group; n = 3; C: control group; U: UV group; T: treatment group).
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