Protective Activity of Alpha-Mangostin against UVB-Induced Injury in HaCaT Cells by Modulating the Ceramide and MAPK and NF-κB Signaling Pathways

Figure 3

α-MG inhibited UVB-induced inflammatory reactions. (a, b) IL-6 and TNF-α expressions were detected by RT-qPCR after α-MG pretreatment and then UVB irradiation. (c) The content of IL-6 in the cell supernatant was detected by ELISA. (d) After α-MG pretreatment and then UVB irradiation, NF-κB and p-NF-κB were detected by western blot. (e, f) GAPDH as an internal control, the expression levels of NF-κB, and p-NF-κB proteins were presented (, , and vs. the control group; , , and vs. the UV group; n = 3; C: control group; U: UV group; T: treatment group).

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