Efficacy for Bone Deterioration in Mice with Osteoporosis and Pharmaceutical Properties of a Novel Compound Calcium Carbonate Granule

Figure 4

Effects of CCCG on the femur microstructure and bone growth of osteoporosis (OP) model mice: (a) images of 2D and 3D reconstruction obtained by micro-CT after the administration of CCCG or CCD. The red arrow represented the X-axis, the blue arrow showed the Y-axis, and the green arrow indicated the Z-axis. Microstructure parameters of BV/TV (b), Tb.Th (c), Tb.Sp (d), Tb.N (e), and Tb.Pf (f) were detected. (g–j) Changes in the biomechanical characteristics of the femur, including maximum load, stiffness, maximum reflection, and frame energy. (k) 36 days after CCCG or CCD administration, H&E staining (40X) was used to observe the pathological changes of the distal femur. Yellow arrows indicated trabeculae; black arrows represented cartilage; green arrows represented bone marrow cavity; purple arrows showed a small amount of new bone, and red arrows represented the cortical bone. Control: normal mice; model: RA-induced OP model mice; CCCG-L: low-dose CCCG-treated OP model mice; CCCG-M: middle-dose CCCG-treated OP model mice; CCCG-H: high-dose CCCG-treated OP model mice; CCD: CCD-treated OP mice. , , and ; n = 6 per group.

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