Research Article

The Effect of Lycium ruthenicum Murry Anthocyanins on the Apoptosis and Proliferation of H9c2 Cell Induced by Hypoxia

Figure 2

LRMA attenuated H9c2 cell apoptosis caused by hypoxia. After exposure of H9c2 cells to 50 μg/mL LRMA incubation for 12 h under normoxia + 24 h under hypoxic condition, Hoechst33343/PI was performed to analyze apoptotic cells. (a) In addition, 50 μg/mL LRMA and 100 μM sal were incubated for 12 h under normoxia + 24 h under hypoxic condition, respectively, and ROS content (b) as well apoptotic rate (c) of H9c2 cells were examined. Then, the Bcl2, Bax, and Bcl2/Bax expressions at mRNA (d) and protein (e) level were detected, respectively. N = 3. and . NS: no significance.