Ursolic Acid Inhibits the Growth of Gastric Cancer by Targeting KLF4/YAP1

Figure 4

KLF4 and TEADs compete for interaction with YAP1 protein. (a) Cell immunofluorescence assay of KLF4 and YAP1 in BGC-823. (b) 1–5: yeast cotransformed with pgadt7 + pgbkt7 (blank control), pgadt7-t + pgbkt7-lam (negative control, large t antigen does not bind to lam protein), pgadt7-t + pgbkt7-P53 (positive control, large t antigen can bind to p53 protein), pgadt7-yap1 + pgbkt7-klf4 (experimental group 1), and pgadt7-yap1 + pgbkt7-tead4 (experimental group 2). Above: five groups of plasmid transfected Y2hgold were coated on the double deficient plate (SD/-Trp/-Leu/AbA/X-α-Gal), below: five groups of plasmids were transfected with Y2hgold and coated on the four deficient plates (SD/-Trp/-Leu/-His/-Ade/AbA/X-α-Gal). (c) The crystal model of KLF4. (d) The score of the KLF4 model according to the GB/VI score function. (e–g) The docking site between KLF4 and YAP1. (h) Co-IP assay indicated binding of KLF4 and YAP1 in BGC-823 cells.

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