Astragaloside IV Suppresses the Effects of Hepatocellular Carcinoma Cells on Proliferation, Angiogenesis, and Invasion in Human Umbilical Vein Endothelial Cells by Controlling Exosomes by Inhibiting Rab27a

Figure 5

AS-IV inhibits the effects of Hep3B and SK-Hep1 cells on HUVECs by inhibiting Rab27a. (a, b) Expression of Rab11, Rab27a, Rab27b, and Rab35 in Hep3B and SK-Hep1 cells. (c) Expression of Rab27a in Hep3B and SK-Hep1 cells. (d, e) Expression of Rab27a in Hep3B cells. (f) Proliferation, (g, h) invasion and (i, j) angiogenesis, and (k) expression of E-cadherin and N-cadherin in HUVECs cocultured with Hep3B or SK-Hep1 cells transduced with Rab27a expression vector before treatment with 50 m AS-IV. Scale bar: 100 m. All data are expressed as the mean ± SD, n = 3.  < 0.01,  < 0.001 vs. control; ^# < 0.05, ^## < 0.01, ^### < 0.001 vs. Hep3B; ^Δ < 0.05, ^ΔΔΔ < 0.001 vs. Hep3B + AS-IV-50.

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