Total Flavone Content of Cydonia oblonga Miller Inhibits Proliferation and Migration of Renal Carcinoma Cells by Inhibiting the S1PR2/FAK Pathway

Figure 3

TFCOM induced apoptosis of 786-O and Renca cells. (a) The apoptotic cell of 786-o cells was examined by the Hoechst 33342 staining assay. (b) Rate of apoptosis cells. (c) Expression of protein associated apoptosis was checked in 786-O cells treated with TFCOM for 48 h by western blotting. Expression of BAX, cleaved-PARP, and cleaved-caspase 3 was upregulated while the ratio of PARP, caspase-3, and expression of Bcl-2 was reduced. Experiments were performed in triplicate. (d) The apoptotic cell of Renca was examined by Hoechst 33342 staining assay. (e) Rate of apoptosis cells. (f) Expression of protein-associated apoptosis was checked in Renca cells treated with TFCOM for 48 h by western blotting. Expression of BAX, p53, cleaved-PARP, and cleaved-caspase 3 was upregulated while the ratio of PARP, caspase-3, and expression of Bcl-2 was reduced. Experiments were performed in triplicate. Changes in the levels of BAX, Bcl-2, cleaved-PARP/PARP, and caspase-3/cleaved-caspase-3 proteins after being normalized to the levels of GAPDH were presented. Data are shown as mean ± SD of three independent experiments. , , and , as compared with the nontreated control.

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