Astragalus Polysaccharides Alleviate Lung Adenocarcinoma Bone Metastases by Inhibiting the CaSR/PTHrP Signaling Pathway

Figure 2

APS promoted OBs differentiation and expression of related genes. (a, b) Osteoblast precursor cells were treated with different concentrations of APS. ALP is a marker for osteoblast activation. ALP staining showed that APS promoted OBs differentiation and this was concentration-dependent (n = 3 per group). (c, d) The related protein expression of OBs differentiation RANKL, OPG, Runx2, and ALP was detected by Western blot after APS treatment. Image J software (NIH) for image processing was used to verify the band intensities as a result of semiquantitative western blots. In the APS treatment group, the protein expression of OPG, Runx2, and ALP was significantly higher than those of the negative control group, while RANKL was significantly lower than that of the negative control group (n = 3 per group). The effect was concentration-dependent. (, , , , the statistical test method was one-way ANOVA).

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