Astragalus Polysaccharides Alleviate Lung Adenocarcinoma Bone Metastases by Inhibiting the CaSR/PTHrP Signaling Pathway

Figure 3

APS promoted A549 cell apoptosis and inhibited A549 cell proliferation and migration. (a, b) CCK-8 was used to detect the proliferation of A549 cells. In the APS-treated group, the proliferation ability of A549 cells was significantly lower than those of the negative control group. The inhibitory effect was concentration-dependent. (c, d) Flow cytometry assay was used to detect cell apoptosis. It showed that APS increased the percentage of apoptotic cells and a higher APS concentration induced a higher percentage of apoptotic cells. (e, f) The cell migration assay showed the effect of APS on migration inhibition in lung adenocarcinoma cells. APS inhibited A549 cell migration and this inhibitory effect was concentration-dependent. (, , , , the statistical test method was one-way ANOVA).

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