Astragalus Polysaccharides Alleviate Lung Adenocarcinoma Bone Metastases by Inhibiting the CaSR/PTHrP Signaling Pathway

Figure 4

APS inhibited the expression of CaSR-involved signaling pathway-related proteins in A549 cells. (a, b) Western blot confirmed the expression of CaSR, PTHrP, and NF-κB. Image J software (NIH) for image processing was used to verify the band intensities as a result of a semiquantitative western blot. The expressions of CaSR, PTHrP, and NF-κB in the APS-treated group were significantly lower than those of the negative control group. The inhibitory effect was concentration-dependent. (c, d) When CaSR was overexpressed, the expression of PTHrP and NF-κB turned out to be high, while APS inhibited the expression of CaSR, as well as PTHrP and NF-κB (). The inhibitory effect was significant between the APS (200 mg/L group) and the negative control group. (, , , , the statistical test method was one-way ANOVA).

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