Prediction and Evaluation of Bioactive Properties of Cowpea Protein Hydrolysates
Table 4
Predicted ASA and secondary structure of bioactive peptides produced from hydrolysis of cowpea protein isolate major proteins (8SG, 7SG3, and VIC).
Enzyme
Protein
Sequence (N° of location)
ASA (nm2)
Secondary structure
IC50 in μM
SASM
SASaa1
SASaa2
SASaa3
SASaa4
Thermolysin
7SG3
LPHH
0.258
0.064
0.356
0.034
0.579
Beta sheet
—
LHR
0.620
0
1.121
0.74
Beta turn; beta hairpin
—
FP
0.828
0.443
1.212
Gamma turn
315
YP
0.252
0.253
0.25
Beta turn
720
IP (1)
0.311
0
0.622
Beta sheet; beta turn
130
IP (2)
0.231
0.394
0.067
Beta sheet
130
IP (3)
0.125
0.064
0.186
Beta sheet; beta turn
130
VG
0.231
0.462
0
Beta sheet
1100
FG
0.000
0
0
Beta sheet
3700
IE
0.404
0
0.807
Beta sheet
—
VE (1)
0.014
0
0.027
Beta sheet
—
VE (2)
0.166
0.053
0.279
Beta sheet
—
LN
0.084
0
0.167
Beta sheet
—
LR (1)
0.188
0.091
0.284
Beta sheet
158
LR (2)
0.084
0
0.167
Beta sheet
158
Thermolysin
8GS
LPHH
0.211
0.186
0.265
0
0.394
Beta sheet
—
FP
1,127
0.693
1.56
Gamma turn
315
YP
0.339
0.257
0.421
Beta turn
720
IP (1)
0.298
0
0.595
Beta turn
130
IP (2)
0.222
0.277
0.167
Beta sheet
130
IP (3)
0.053
0
0.106
Beta turn
130
FG (1)
0.046
0.091
0
Beta sheet
3700
FG (2)
0.749
1,431
0.067
Beta turn
3700
LG
0.610
0.952
0.267
Beta turn
8800
VR
1.594
0.939
2.249
Beta turn
52.80
AR
0.000
0
0
Beta sheet
95.50
VE (1)
0.000
0
0
Beta sheet
—
VE (2)
0.372
0.08
0.664
Beta sheet
—
LN
0.114
0.027
0.201
Beta sheet
—
YE
0.276
0.353
0.198
Beta sheet
630.90
LR (1)
0.146
0
0.292
Beta sheet
158
LR (2)
0.154
0
0.307
Beta sheet
158
Thermolysin
VIC
LPHH
0.214
0.208
0.33
0.034
0.284
Beta sheet
—
LHE
0.100
0.053
0.08
0.167
Beta sheet
—
FP
1.107
0.708
1.505
Gamma turn
315
YP
0.241
0.295
0.186
Beta turn
720
VK
0.053
0
0.106
Beta sheet
13
IP
0.053
0
0.106
Beta sheet; beta turn
130
VP (1)
0.000
0
0
Beta turn
420
VP (2)
0.191
0.314
0.067
Beta sheet; beta turn
420
FG (1)
0.853
1,639
0.067
Beta turn
3700
FG (2)
0.000
0
0
Beta sheet
3700
LG
0.336
0.671
0
Beta turn
8800
IE
0.143
0
0.285
—
—
VE
0.000
0
0
Beta sheet
—
LN
0.064
0.033
0.095
Beta sheet
—
LR (1)
0.186
0.08
0.292
Beta sheet
158
LR (2)
0.080
0
0.159
Beta sheet
158
Alcalase
VIC
VKL
0.020
0
0.027
0.033
Beta sheet
—
KAI
0.338
0.897
0
0.117
Beta turn; beta sheet
—
VF (1)
0.032
0
0.064
Beta sheet
9.20
VF (2)
0.000
0
0
Beta sheet
9.20
VP
0.226
0.352
0.1
Beta sheet
420
GI
0.050
0.067
0.033
Beta turn
1200
NF
0.040
0.027
0.053
Beta sheet
46.30
DF
0.755
0.786
0.724
Helix
363.10
IL
0.080
0.159
0
Beta sheet
54.95
Alcalase
7SG3
VF (1)
0.000
0
0
Beta sheet
9.20
VF (2)
0.000
0
0
Beta sheet
9.20
AIP
0.165
0
0.394
0.1
Beta sheet
670
AF
0.033
0.027
0.038
Beta sheet
190
GS
0.040
0.08
0
Beta turn
3800
NF
0.110
0.193
0.027
Beta sheet
46.30
DF
0.689
0.771
0.606
Helix
363.10
IL (1)
0.192
0.383
0
Beta sheet
54.95
IL (2)
1,045
0.894
1.195
Beta turn
54.95
PHY
0.367
0.462
0.027
0.613
Beta sheet
—
KAI
0.380
0.89
0
0.25
Beta turn
—
Alcalase
8SG
PHY
0.304
0.489
0.053
0.369
Beta sheet
—
KAI
0.383
0.91
0
0.239
Beta turn
—
VF (1)
0.000
0
0
Beta sheet
9.20
VF (2)
0.000
0
0
Beta sheet
9.20
AIP
0.126
0
0.277
0.1
Beta sheet
670
AF
0.038
0
0.075
Beta sheet
190
GI
0.318
0.067
0.569
Beta turn
1200
GS
0.014
0
0.027
Beta turn
3800
DF
0.763
0.711
0.815
Helix
363.10
IL (1)
0.102
0.204
0
Beta sheet
54.95
IL (2)
0.595
0.531
0.659
Beta turn
54.95
Trypsin
8GS
GR
0.754
0.027
1.481
Gamma turn
3200
AR
1.154
0.609
1.698
Beta turn
95.50
Trypsin
VIC
Trypsin
7SG3
GR
0.171
0.027
0.314
Beta turn
3200
SASaai, solvent accessible surface of the ith amino acid; SASM, mean solvent accessible surface of the peptide; the IC50 values were provided by the BIOPEP database.
