Immunomodulatory Effects of Nervonic Acid in RAW264.7 Macrophages

Figure 5

Effects of NA on the expression of NF-κB pathway-related proteins and nuclear translocation of in RAW264.7 cells. (a) RAW264.7 cells (2 × 10⁶ cells/well) were treated with three NA concentrations (12.5, 25, and 50 μmol/L) or with LPS (1 μg/mL) in 6-well flat-bottom plates for 24 h. CTL; control, LPS; lipopolysaccharide. Western blots showed the expression of the representative proteins. The bar graphs, respectively, showed density ratios of TLR4 (b) and p-IκBα to total IκBα (c) as analyzed using ImageJ software. Nuclear NF-κB translocation (d) was observed by CLSM (×200, scale bar = 100 μm). Relative fluorescence intensity (e). Data are shown as the mean ± S.D. of three independent experiments. and vs. the control group.

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