Morroniside Delays NAFLD Progression in Fructose-Fed Mice by Normalizing Lipid Metabolism and Inhibiting the Inflammatory Response

Figure 3

Morroniside enhances hepatic lipogenesis and impairs β-oxidation in mice. (a)–(d) The mRNA expression of Pgc1β, Srebp1, Fasn, and Acc from the con or fructose-fed mice with intragastric administration of either vehicle or morroniside, respectively. (e, f) Representative immunoblots and quantification of PGC1β, p-SREBP-1 (precursor), n-SREBP-1 (nuclear), FASN, and ACC in liver. (g) Representative images of immunohistochemistry assay of FASN and ACC in liver. (h) The mRNA expression of Pparα and Cpt1α from the con or fructose-fed mice with intragastric administration of either vehicle or morroniside, respectively. (i, j) Representative immunoblots and quantification of PPARα and CPT1α in liver. Mean ± S.D., n = 8 for RT-qPCR quantification analysis and n = 3 for liver protein expression analysis. ^##, ^### vs. the con group; , , vs. the fructose-fed group. Con: control.

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