Nuciferine Promotes Longevity and Fitness in Caenorhabditis elegans through the Regulation of the Insulin/IGF-1 Signaling Pathway

Figure 4

Nuciferine-activated DAF-16/FOXO and its downstream genes and proteins. (a) Typical fluorescence images of DAF-16::GFP distribution in TJ356: cytosolic, intermediate, and nuclear. (b) Nuciferine promoted the nuclear translocation of DAF-16. (c) The mRNA expression of daf-2, age-1, sgk-1, daf-16, and its downstream genes. (d) The fluorescent images and quantification of SOD-3::GFP expression in CF1553 strain. (e) The fluorescent images and quantification of HSP-16.2::GFP expression in CL2070 strain. Data were represented as mean ± SD, n = 3. Statistical significance was obtained at , , , and by the t-test. ns: not significant.

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