Nuciferine Promotes Longevity and Fitness in Caenorhabditis elegans through the Regulation of the Insulin/IGF-1 Signaling Pathway

Table 2

Effects of nuciferine on the lifespan of mutants related to the IIS pathway.

Strains	Group	Mean lifespan (days ± SEM)	Change (%)	Number of worms	P value
daf-2(e1370)III
	Control	26.51 ± 0.70	—	156
	Nuciferine 100 μM	27.54 ± 0.65	−3.92%	182	0.622 (ns)
age-1(hx546)II
	Control	24.56 ± 0.61	—	135
	Nuciferine 100 μM	25.70 ± 0.78	4.66%	90	0.2287 (ns)
sgk-1(ok538)X
	Control	22.66 ± 0.64	—	151
	Nuciferine 100 μM	23.45 ± 0.60	3.48%	113	0.9182 (ns)
daf-16(mu86)I
	Control	14.74 ± 0.31	—	129
	Nuciferine 100 μM	14.93 ± 0.32	1.29%	137	0.5786 (ns)
skn-1(zu67)IV
	Control	15.66 ± 0.43	—	169
	Nuciferine 100 μM	14.88 ± 0.38	−4.96%	172	0.2270 (ns)
hsf-1(sy441)I
	Control	15.02 ± 0.40	—	126
	Nuciferine 100 μM	15.41 ± 0.38	2.61%	125	0.6143 (ns)

P values were calculated by the log-rank test using the Kaplan–Meier survival analysis (). ns, not significant. Mean lifespan values were calculated by the log-rank test and presented as mean ± SEM by SPSS.