MAPK and PI3K/Akt Signalling Pathways Potentially Modulate the Enhancing Effect of Black Tea Extracts on Endo180 Expression and Collagen Internalisation

Figure 2

Correlation between Oregon Green™ 488-conjugated gelatin (OG-gelatin) concentration and fluorescence intensity and competitive inhibitory effect of gelatin on cellular internalisation of OG-gelatin. (a) The increase in fluorescence intensity slowed when the OG-gelatin concentration was above 6.3 μg/mL. Human dermal fibroblasts (HDFs) were treated with the indicated concentration of OG-gelatin for 2 h. OG-gelatin internalisation was measured as described in the “Collagen Internalisation Measurement” section. Results are expressed as mean ± standard deviation of three independent experiments. Different letters denote significant differences at . (b) Gelatin competitively inhibited cellular internalisation of OG-gelatin in HDFs. HDFs were treated with a fixed concentration of OG-gelatin and the indicated concentrations of gelatin for 2 h. OG-gelatin internalisation was measured and calculated as described in the “Collagen Internalisation Measurement” section. Results are expressed as mean ± standard deviation of three independent experiments. Different letters denote significant differences at .

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