MAPK and PI3K/Akt Signalling Pathways Potentially Modulate the Enhancing Effect of Black Tea Extracts on Endo180 Expression and Collagen Internalisation

Figure 4

Effects of black tea extract (BTE) on Endo180 expression in human dermal fibroblasts (HDFs). (a) BTE increased Endo180 mRNA expression in HDFs. HDFs were treated with the indicated concentrations of BTE for 1 day. Quantitative real-time PCR was conducted as described in the “Real-Time PCR” section. Endo180 mRNA expression in each treatment group was calculated based on that in the untreated control group and is expressed as mean ± standard deviation of three independent experiments. Different letters denote significant differences at . (b) BTE induces Endo180 protein expression in HDFs. HDFs were treated with the indicated concentrations of BTE for 1 day. Enzyme-linked immunosorbent assay was conducted as described in the “Enzyme-Linked Immunosorbent Assay (ELISA)” section. Endo180 protein expression in each treatment group is calculated as a percentage compared with that in the untreated control group and is expressed as mean ± standard deviation of three independent experiments. Different letters denote significant differences at .

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