Cyanidin-3-O-Glucoside Combined with PGC1α Inhibitor Promotes Cancer Cell Death by Inducing Excessive Levels of ROS in Cervical Squamous Cell Carcinoma

Figure 6

(a) The effect of C3G (8 μM), NAC (10 μM), SR-18292 (10 μM), and their combination treatment on cell proliferation was determined by EdU/Hoechst 33342 staining. Scare bar: 50 μm. (b) Mitochondrial membrane potential assay to detect the effect of C3G alone (8 μM), SR-18292 alone (10 μM), and the combination of C3G (8 μM) and SR-18292 (10 μM) on the Δψm reduction of HCC94 and SiHa cells. Fluorescence images were obtained after 48 h treatment, and representative images of JC-1 monomer (green), JC-1 aggregate (red), and merge were shown. Scare bar: 20 μm.

(a)

(b)