Immune-Enhancing Activity of Vitis coignetiae Extract via Increasing Cytokine and Natural Killer Cell Activity in Splenocytes and Cyclophosphamide-Induced Immunosuppressed Rats

Figure 8

Effect of Vitis coignetiae extract (VCE) on natural killer (NK) cell activity in cyclophosphamide (Cy)-treated Wistar rat splenocytes and Cy-induced immunosuppressed rats. Wistar rat splenocytes were treated with VCE (0–300 μg/mL) for 24 h and Cy-induced immunosuppressed rat splenocytes were treated with Cy (5 mg/kg/d), oral VCE (0, 50, 250, and 500 mg/kg/d), and HemoHIM (positive control; 1,000 mg/kg/d) once daily for four weeks, after which spleens were collected for analysis. Splenocytes were cocultured with target cells (AR42J) in 96-well plates. Then, the cells were incubated for 24 h in a 5% CO₂ incubator with a ratio of effector to target cells of 20 : 1. NK cell activity was calculated as the survival rate of AR42J cells compared to those of the control group. Bars labeled with different superscripts have significantly different values ().

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