Neuroprotective Effect of Fructus Ligustri Lucidi in Spinal Cord Neuron Injury during H₂O₂-Mediated Oxidative Stress

Figure 2

Effect of FLL extract on ROS accumulation and iNOS expression. (a) Flow cytometric dot plot depicting the assessment of reactive oxygen species (ROS) in spinal cord neurons using DCFDA. (b) MAP2 (red) and iNOS (green) expression immunocytochemistry images with confocal microscopy. White scale bar = 200 μm. Yellow scale bar = 50 μm. (c) Flow cytometric quantification of ROS-positive cells, challenged with 500 μM H₂O₂. (d) Quantitative evaluation of each group’s relative iNOS expression fluorescence intensity. (e) Expression of the iNOS gene was measured in spinal cord neurons after cotreatment with FLL and H₂O₂ for 24 h. Data are expressed as means ± SEM. Significant differences are indicated as ^#### compared with the blank group. , , and vs. the H₂O₂ group were analyzed via one-way ANOVA with Tukey’s post hoc test.

(a)

(b)

(c)

(d)

(e)