Neuroprotective Effect of <i>Fructus Ligustri</i> Lucidi in Spinal Cord Neuron Injury during H<sub>2</sub>O<sub>2</sub>-Mediated Oxidative Stress

<div>Antioxidant effect of FLL extract by upregulation of the Nrf2/HO-1 pathway. (a and b) Nrf2 and HO-1 immunocytochemistry images with confocal microscopy. White scale bar = 200 <i>μ</i>m. Yellow scale bar = 50 <i>μ</i>m. (c) Quantitative evaluation of each group’s relative Nrf2 expression fluorescence intensity. (d) Expression of the <i>Nrf2</i> gene was measured in spinal cord neurons after cotreatment with FLL and H<sub>2</sub>O<sub>2</sub> for 24 h. (e) Quantitative evaluation of each group’s relative HO-1 expression fluorescence intensity. (f) Expression of the <i>HO-1</i> gene was measured in spinal cord neurons after cotreatment with FLL and H<sub>2</sub>O<sub>2</sub> for 24 h. Data are expressed as means ± SEM. Significant differences are indicated as <sup>#</sup><svg height="11.7782pt" id="M10" style="vertical-align:-3.42938pt" version="1.1" viewbox="-0.0498162 -8.34882 44.5261 11.7782" width="44.5261pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 -12 283 -12C351 3 429 48 483 105C543 168 570 242 570 304ZM482 289C482 161 380 33 304 33C278 33 248 51 233 69L303 396C326 400 352 403 369 403C428 403 482 380 482 289Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.342,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"></path></g><g transform="matrix(.013,0,0,-0.013,22.605,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 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205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. the H<sub>2</sub>O<sub>2</sub> group were analyzed via one-way ANOVA with Tukey’s post hoc test.</div>

Journal of Food Biochemistry

fig3

Figure 3

Figure 3: Neuroprotective Effect of <i>Fructus Ligustri</i> Lucidi in Spinal Cord Neuron Injury during H<sub>2</sub>O<sub>2</sub>-Mediated Oxidative Stress 

Journal of Food Biochemistry

Neuroprotective Effect of Fructus Ligustri Lucidi in Spinal Cord Neuron Injury during H2O2-Mediated Oxidative Stress

Figure 3

Neuroprotective Effect of Fructus Ligustri Lucidi in Spinal Cord Neuron Injury during H₂O₂-Mediated Oxidative Stress