Low-Molecular-Weight Hydrolysate from Black Goat Extract Had Antioxidative and Anti-Inflammatory Effects in Macrophage Cells via Inhibition of MAPKs and NF-κB Pathways
Table 2
Amino acid sequences of peptides in F5 and their HAA composition (%).
No.
Sequence
MW (Da)
Probability (%)
HAA (%)
1
GVXGPXGAVGPA
1006.51
99.00
83.33
2
GRPGEVGPPGPPGPAGE
1574.73
99.00
82.35
3
VGPPGPSGNAGPXGPXGPA
1643.74
99.00
78.95
4
GAPGADGPAGAXGTPGPQ
1489.68
99.00
77.78
5
GPXGAXGAXGAXGPVGPA
1486.71
99.00
77.78
6
GVQGPXGPAGPR
1104.57
99.00
75.00
7
VGPXGPXGPAGE
1062.50
99.00
75.00
8
KGAPGADGPAGAXGTPGPQ
1617.77
99.00
73.68
9
GFAGPXGADGQPGAK
1341.63
99.00
73.33
10
GPXGPAGPAGERGEQGPA
1616.75
99.00
72.22
11
GPXGPSGNAGPXGPXGPA
1528.68
99.00
72.22
12
GPXGPXGPXGPXGPPSGG
1538.70
99.00
72.22
13
GPSGPQGPSGPXGP
1203.55
99.00
71.43
14
VGPXGPSGNAGPXGPXGPAGK
1812.86
99.00
71.43
15
GIQGPXGPAGEEG
1180.54
99.00
69.23
16
GRXGEVGPXGPXGPAG
1445.69
99.00
68.75
17
GAPGDKGETGPSGPAGPTGAR
1851.87
99.00
66.67
18
GPSGPQGPSGPXGPK
1331.65
99.00
66.67
19
GFXGSDGVAGPK
1103.52
99.00
66.67
20
GIQGPXGPAGEE
1123.51
99.00
66.67
21
GVXGDLGAXGPS
1054.49
99.00
66.67
22
QGLXGPAGPXGE
1107.52
99.00
66.67
23
GRXGEVGPXGPXGPA
1388.67
99.00
66.67
24
GLXGPXGAXGPQ
1091.52
99.00
66.67
25
GIQGPXGPAGEEGK
1308.63
99.00
64.29
26
GPXGLAGPXGESGR
1279.62
99.00
64.29
27
TGPXGPAGQDGRXGPXGPXGAR
2055.97
99.00
61.90
28
GAXGDKGETGPSGPAGPT
1567.71
99.00
61.11
29
XGDKGETGPSGPAGPTGA
1549.70
99.00
61.11
30
GSEGPQGVRGEXGPXGPA
1677.76
99.00
61.11
31
GRXGEVGPXGPXGPAGEK
1702.83
99.00
61.11
32
GIQGPXGPAGEEGKR
1464.73
99.00
60.00
33
GAXGDRGEXGPXGPA
1378.61
99.00
60.00
34
AGKXGEQGVXGDLGAXGPS
1737.82
99.00
57.89
35
QGPXGEXGEXGASGPMGPR
1821.80
99.00
57.89
36
QGPXGPXGSXGEQGPSGASGPAGPR
2243.02
99.00
57.14
37
QGPXGEXGEXGASGPM
1494.59
99.00
56.25
38
GEXGSXGENGAPGQMGPR
1742.72
99.00
55.56
39
GSXGEAGRXGEAGLXGAK
1654.79
99.00
55.56
40
QGPXGPXGSXGEQGPSGA
1620.70
99.00
55.56
41
SGLQGPXGPXGSXGEQGPS
1749.78
99.00
52.63
42
QGPXGPXGSXGEQGPS
1475.62
99.00
50.00
43
GAPGFPGAR
860.42
97.93
50.00
MW, observed molecular weight; HAA, hydrophobic amino acids; X, hydroxyproline. Hydrophobic amino acids (%) is the percentage of hydrophobic residues (G, V, L, I, P, F, M) in the peptide sequence.