Cloning, Characterization, and Functional Expression of Phospholipase Dα cDNA from Banana (Musa acuminate L.)

Figure 3

Expression of gene MaPLDα in different banana organs. Equivalent cDNA amounts from different banana organs, that is, floral buds (Fb), flowers (Flo), green leaves (Gl), senescent leaves (Sl), pseudo stem (Ps), stem (St), developing fruits at the broken bud stage (Df1), 30 days after the broken bud stage (Df2), and 60 days after the broken bud stage (Df3), and mature fruits (Mf), were analyzed by semiquantitative PCR as described in Materials and Methods, using primers specific for MaPLDα and a housekeeping gene, actin, as an internal control. Expression levels are presented relative to that of senescent leaves, with a standard deviation ().