Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures

Figure 1

Optimal reaction condition and primers used in the LAMP assay. Amplified products were judged by color change. A positive reaction was indicated by a color change from orange to green. Tubes: 1–6, primers set 1 (60°C–65°C); 7–12, primers set 2 (60°C–65°C); 13–18, primers set 3 (60°C–65°C); 19–24, primers set 4 (60°C–65°C). The color of the primers of set 2 was greener than that of the others, especially at 62°C.